스토어 정보 |
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상품명 | [MBCELL] PALCAM Agar (500g) (008532) |
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자체상품코드 | B-008532 |
판매가 | ₩189,200 (VAT 포함) |
수량 | |
적립금 | 1,890원 |
★ 기타 | MBCELL 주문시 반품 및 교환 불가 (본사 지침사항) |
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*Equally use with MFDS (MB-P1330K).
CONTENTS (Liter) | Peptone | 23.0 g |
Mannitol | 10.0 g | |
Sodium Chloride | 5.0 g | |
Starch | 1.0 g | |
Ferric Ammonium Citrate | 0.5 g | |
Esculin | 0.8 g | |
Dextrose | 0.5 g | |
Lithium Chloride | 15.0 g | |
Phenol Red | 0.08 g | |
Agar | 13.0 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 68.88 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Aseptically add 2 vials of PALCAM Listeria supplement (MB-P1826). Mix well. Dispense in petri dishes.
1 vial contents (each vial is sufficient for 500mL of medium) | |
Polymyxin B | 0.005 g |
Ceftazidime | 0.01 g |
Acriflavine HCl | 0.0025 g |
PALCAM (Polymyxin Acriflavin LiCl Ceftazidime Esculin Mannitol) Agar is a medium used for the isolation and detection of Listeria monocytogenes. Peptone provides source of nutrients for the organisms. Dextrose, starch and mannitol are the carbohydrate and energy sources. Sodium chloride maintains osmotic balance. Phenol red is the pH indicator dye that exhibits changes in the pH of the medium. Listeria monocytogenes hydrolyzes esculin to form esculetin and dextrose. Esculetin reacts with ammonium ferric citrate and forms a brown-black complex seen as a black halo around colonies. Listeria monocytogenes does not ferment mannitol but contaminants such as Enterococci and Staphylococci ferment mannitol and is indicated by color change from red
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 30 °C for 24 - 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light yellow to pink
Prepared medium
Appearance: clear with no precipitate
Color: red
Incubation conditions: 30 °C / 24 - 48 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Listeria monocytogenes | 19111 | 50-100 | good | gray colonies/ black halo |
Listeria monocytogenes | 13932 | 50-100 | good | gray colonies/ black halo |
Escherichia coli | 25922 | ≥10³ | inhibited | - |
Enterococcus faecalis | 29212 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.
1. Van Netten, P. et al.,(1989). J. of Food Microbiol. 8:299-317.
2. AFNOR. (1993). Food Microbiology – “Detection of Listeria monocytogenes”. IDF Provisional International Standard n° 143. International Dairy Federation, Brussels.
3. Refer to the MFDS.
Cat. No : MB-P1330 PALCAM (Polymyxin Acriflavin LiCl Ceftazidime Esculin Mannitol) Agar | 500 G |
( 출처 : 기산바이오 )
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